Epidermal cell cohesion and pemphigus
Die Studien zur Pathogenese des Pemphigus wird von der DFG im Rahmen der FOR 2497 PEGASUS gefördert
Jens Waschke (group leaders)
Daniela Kugelmann (group leader, research focus: Dsg1-mediated cell adhesion)
Anna Sigmund (group leader, research focus: Dsg-mediated protein complexes)
Thomas Schmitt (group leader, research focus: Dsg-mediated signaling)
Franziska Vielmuth (group leader, research focus: Biophysical characterization of keratinocyte adhesion)
Desalegn Egu (research fellow)
Markus Winkler (MD student)
Sophia Engelmeyer (MD student)
Maresa Anders (MD student)
Vincent Braun (MD student)
Clara Lampe (MD student)
Martina Hitzenbichler (technician)
Sabine Mühlsimer (technician)
Silke Gotschy (technician)
The epidermis represents the outermost barrier sealing our body against the environment. The integrity of these stratifying cell layers depends on desmosomes, specific intercellular junction complexes designed to tether adjacent keratinocytes together. This enables the epidermis to withstand the high mechanical forces this tissue constantly is exposed to. The core of desmosomes is composed of the cadherin-type transmembrane adhesion molecules desmogleins and desmocollins. These molecules are intracellularly linked to the intermediate filament network by the adapter proteins plakoglobin, plakophilins and desmoplakin (Fig. 1). .
Fig. 1 Schematic of a desmosome
Specifically desmogleins are known to exist “free” on the membrane without attachment in the desmosome. These molecules are connected to a variety of signaling molecules such as Rho-GTPases, Src and p38MAPK. By regulating the activity of these molecules, extradesmosomal desmogleins serve as signaling hubs to modulate cellular behavior (Fig. 2).
Fig. 2 Extradesmosomal desmoglein molecules serve as signaling hubs
The importance of desmosomal adhesion becomes evident by the autoimmune disease pemphigus. The main clinical variant is pemphigus vulgaris. Patients develop autoantibodies against the desmoglein isoforms 3 and 1 (Dsg3 and Dsg1) resulting in loss of desmosomal function. This loss of cell cohesion leads to cleft formation within the epidermis and the mucosa of the oral cavity. Macroscopically, blisters are visible which rupture rapidly (Fig.3). Without immunosuppression, the disease is lethal.
Fig. 3: Pemphigus
Binding of pemphigus autoantibodies leads to altered intracellular signaling, at least in part following initial loss of Dsg3 interaction (Fig. 4). This induces (i) loss of Dsg3 and other desmosomal molecules from the cell surface and (ii) uncoupling of the intermediate filaments from the desmosome. These two effects are thought to represent the main mechanisms of loss of cell-cell adhesion and ultimately blister formation. Currently, we focus on signaling patterns induced by autoantibodies from patients with different pemphigus phenotypes.
Fig. 4: Mechanisms of blister formation in pemphigus
Within the project we investigate the regulation of desmosomal adhesion. Specifically, we address how desmosomes are assembled and disassembled and which mechanisms and signals are involved in these processes. Furthermore, we aim to better understand the mechanisms that lead to loss of cell cohesion and blister formation in pemphigus to identify novel targets as potential treatment options for this life-threatening disease.
We combine state-of-the-art biochemical and molecular biology techniques with morphological assessment of desmosomes and their turnover (using live cell imaging, confocal microscopy, transmission and scanning electron microscopy). To correlate this with the adhesive function of desmosomes, we apply adhesion assays and biophysical techniques such as atomic force microscopy and optical tweezers (see here).
Recent publications (selection since 2013):
Spindler V, Rötzer V, Dehner C, Kempf B, Gliem M, Radeva M, Hartlieb E, Harms GS, Schmidt E, Waschke J (2013). Peptide-mediated desmoglein 3 crosslinking prevents pemphigus vulgaris autoantibody-induced skin blistering; J Clin Invest, 123(2):800–811
Hartlieb E, Kempf B, Partilla M, Vigh B, Spindler V, Waschke J.(2013) Desmoglein 2 is less important than desmoglein 3 for keratinocyte cohesion. PLoS One. 2013;8(1):e53739. doi: 10.1371/journal.pone.0053739. Epub 2013 Jan 11.
Dehner C, Rötzer V, Waschke J, Spindler V (2014). A desmoplakin point mutation with enhanced keratin association ameliorates pemphigus vulgaris autoantibody-mediated loss of cell cohesion. Am J Pathol, 184(9):2528-36.
Hartlieb E, Rötzer V, Radeva M, Spindler V, Waschke J (2014). Desmoglein 2 compensates for desmoglein 3 but does not control cell adhesion via regulation of p38-mitogen-activated protein-kinase in keratinocytes. J Biol Chem, 289(24):17043-17053
Rötzer V, Breit A, Waschke J, Spindler V (2014). Adducin is required for keratinocyte cohesion . J Biol Chem, 289(21):14925-40
Spindler V, Dehner C, Hübner S, Waschke J (2014). Plakoglobin but not desmoplakin regulates keratinoycte cohesion via p38MAPK signaling. J Invest Dermatol, 134(6):1655-64
Vielmuth F, Waschke J, Spindler V. (2015). Loss of Desmoglein Binding Is Not Sufficient for Keratinocyte Dissociation in Pemphigus. J Invest Dermatol. 2015 Dec;135(12):3068-3077. doi: 10.1038/jid.2015.324. Epub 2015 Aug 19.
Rötzer V, Hartlieb E, Vielmuth F, Gliem M, Spindler V, Waschke J. (2015) E-cadherin and Src associate with extradesmosomal Dsg3 and modulate desmosome assembly and adhesion. Cell Mol Life Sci. 2015 Dec;72(24):4885-97. doi: 10.1007/s00018-015-1977-0. Epub 2015 Jun 27.
Rötzer V, Hartlieb E, Winkler J, Walter E, Schlipp A, Sardy M, Spindler V, Waschke J. (2016) Desmoglein 3-Dependent Signaling Regulates Keratinocyte Migration and Wound Healing. J Invest Dermatol. 2016 Jan;136(1):301-10. doi: 10.1038/JID.2015.380.
Egu DT, Walter E, Spindler V, Waschke J (2017) Inhibition of p38MAPK signaling prevents epidermal blistering and alterations of desmosome structure induced by pemphigus autoantibodies in human epidermis. Br J Dermatol. (2017) 177:1612–8. doi: 10.1111/bjd.15721.
Walter E, Vielmuth F, Rotkopf L, Sárdy M, Horváth ON, Goebeler M, Schmidt E, Eming R, Hertl M, Spindler V, Waschke J (2017) Different signaling patterns contribute to loss of keratinocyte cohesion dependent on autoantibody profile in pemphigus. Sci Rep. (2017) 7:3579. doi: 10.1038/s41598-017-03697-7.
Egu DT, Kugelmann D, Waschke J. Role of PKC and ERK Signaling in Epidermal Blistering and Desmosome Regulation in Pemphigus. Front Immunol. 2019 Dec 6;10:2883. doi: 10.3389/fimmu.2019.02883. eCollection 2019.
Kugelmann D, Radeva MY, Spindler V, Waschke J. (2019), Dsg1 deficiency causes lethal skin blistering. J Invest Dermatol. 2019 Jan 17. pii: S0022-202X(19)30017-X. doi: 10.1016/j.jid.2019.01.002.
Kugelmann D, Rötzer V, Walter E, Egu DT, Fuchs MT, Vielmuth F, Vargas-Robles H, Schnoor M, Hertl M, Eming R, Rottner K, Schmidt A, Spindler V, Waschke J. Role of Src and Cortactin in Pemphigus Skin Blistering. Front Immunol. 2019 Apr 4;10:626. doi: 10.3389/fimmu.2019.00626. eCollection 2019.
Radeva MY, Walter E, Stach RA, Yazdi AS, Schlegel N, Sarig O, Sprecher E, Waschke J. ST18 Enhances PV-IgG-Induced Loss of Keratinocyte Cohesion in Parallel to Increased ERK Activation. Front Immunol. 2019 Apr 17;10:770. doi: 10.3389/fimmu.2019.00770. eCollection 2019.
Egu DT, Sigmund AM, Schmidt E, Spindler V, Walter E, Waschke J. A new ex vivo human oral mucosa model reveals that p38MAPK inhibition is not effective in preventing autoantibody-induced mucosal blistering in pemphigus. Br J Dermatol. 2020;182(4):987‐994.